REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
Strains used in this study are listed in Table S2	This paper.	N/A
Chemicals, peptides, and recombinant proteins
Meta-bromo-thiolactone (mBTL)	Synthesized by Wuxi AppTec, gift from the Bassler lab (McCready et al.1)	N/A
Isopropyl-β-D-thiogalactopyranoside	GoldBio	CAS #: 367-93-1
N-butyryl-L-homoserine lactone (C4HSL)	Cayman Chemical	Item #: 10007898
N-(3-oxododecanoyl)-L-homoserine lactone (3OC12HSL)	Cayman Chemical	Item #: 10007895
L-arabinose	Millipore Sigma	CAS #: 5328-37-0
Fluorinated Fos-choline-8	Anatrace	Item #: F300F
Critical commercial assays
SuperScript III Reverse Transcriptase kit	ThermoFisher	Item #: 18080044
SYBR™ Select Master Mix	Applied Biosystems	Item #: 4472903
Deposited data
PqsE-RhlR:mBTL	This paper.	PDB: 8DQ0
PqsE-RhlR:mBTL	This paper.	EMD-27645
PqsE-RhlR:mBTL	This paper.	EMPIAR-11247
PqsE-RhlR:mBTL-DNA	This paper.	PDB: 8DQ1
PqsE-RhlR:mBTL-DNA	This paper.	EMD-27646
PqsE-RhlR:mBTL-DNA	This paper.	EMPIAR-11247
Experimental models: Organisms/strains
Pseudomonas aeruginosa: strain UCBPP-PA14	ATCC	BAA-1744
Escherichia coli: strain BL21 (DE3)	New England Biolabs	Item #: C2527H
Oligonucleotides
Primers used in this study are listed in Table S3.	This paper	N/A
Recombinant DNA
Plasmids used in this study are listed in Table S2.	This paper.	N/A
Software and algorithms
SerialEM	Mastronarde2, Schorb et al.3	https://bio3d.colorado.edu/SerialEM/
cryoSPARC	Zivanov et al.4	https://cryosparc.com/
MotionCor2	Zheng et al.5	https://emcore.ucsf.edu/ucsf-software
RELION	Zivanov et al.4	https://relion.readthedocs.io/en/release-4.0/
Phenix	Liebschner et al.6	https://phenix-online.org/
Coot	Emsley et al.7	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Other
300-mesh gold-support grids	Quantifoil	Item #: R1.2/1.3
Ni-NTA resin	Qiagen	Item #: 30210