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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Nat Microbiol. 2022 Oct 31;7(12):1956–1966. doi: 10.1038/s41564-022-01243-4

Extended Data Fig. 3. Sequence alignment of wild type ΦKZ and three escape mutants at the engineered genomic site.

Extended Data Fig. 3

Escape mutants were isolated and verified by PCR and sequencing. The WT orf120 sequence is highlighted in blue and the downstream region is highlighted in grey. The stop codon (TGA) of orf120 is highlighted in green and Escape mutant #3 reconstitutes it to TAG. The sequence in the red frame matches the spacer sequence of the crRNA that was used to target and eliminate WT phages. Deletions were indicated by dashed lines and their corresponding numbers of absent base pairs.