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. 2022 Nov 10;54(11):1927–1939. doi: 10.1038/s12276-022-00879-w

Fig. 2. SA inhibits TNF-α-induced chondrocyte inflammatory factor expression and extracellular matrix degeneration.

Fig. 2

Chondrocytes were stimulated with TNF-α and then treated with or without 10 μM SA for 48 h. a Relative mRNA expression levels of IL1β, IL-6, MMP13, COL2A1 and ACAN were detected by reverse transcription-quantitative polymerase chain reaction (n = 3, one-way ANOVA). b Expression of active MMP13 protein was reduced by the chondrocytes when stimulated with SA (n = 3, one-way ANOVA). c WB analysis for detecting the cartilage matrix markers MMP13, ACAN and COL2A1 in different groups (i). Quantitative analysis of MMP13 (ii), ACAN (iii), and COL2A1 (iv) at the protein level. GAPDH was used as a reference protein (n = 3, one-way ANOVA). The data are expressed as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, and ns not significant.