| Reagent | Amount |
|---|---|
| Digested and purified pAAV-U6-gRNA-CBh-mCherry | 50 ng |
| Diluted annealed oligos | 1 μL |
| 10× T4 ligation buffer (NEB) | 1 μL |
| T4 ligase (NEB) | 1 μL |
| ddH2O | Up to 10 μL |
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