| Reagent | Amount |
|---|---|
| 2× Phanta Max Buffer | 25 μL |
| dNTP Mix (10 mM each) | 1 μL |
| 100 ng/μL template plasmid containing EMRE cDNA | 1 μL |
| 10 μM SLIC-EMRE-F | 1 μL |
| 10 μM SLIC-EMRE-R | 1 μL |
| Phanta Max Super-Fidelity DNA Polymerase (Vazyme) | 1 μL |
| ddH2O | Up to 50 μL |
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