Table 5.
Changes in the MIC of tetracyclines and the information of isolates used in the cloning experiments in this study.
| Isolates | MIC (μg/mL) | Description | ||
|---|---|---|---|---|
| ERV | OMC | TGC | ||
| JP3936 | 0.12 | 2 | 0.25 | OMC-R and ERV/TGC-S strain, with frameshift mutation of mepB |
| JP4612 | 2 | 4 | 2 | Tigecyclines-resistant, with frameshift mutation in mepB |
| JP4200 | 0.06 | 0.5 | 0.12 | Tigecyclines-susceptible-S, without frameshift mutation in mepB |
| E. coli ATCC 25922 | 0.12 | 1 | 0.12 | Used as a control strain |
| E. coli DH5α | 0.06 | 0.5 | 0.12 | Used as a host for the PCR product clone |
| pUCP24/DH5α | 0.06 | 0.5 | 0.12 | E. coli DH5α carrying cloning expression vector pUCP24 |
| pUCP24-mepB/DH5α-JP3936 | 0.06 | 2 | 0.12 | mepB of JP3936 was cloned into the expression vector pUCP24 and transformed into E. coli DH5 α |
| pUCP24-mepB/DH5α-JP4612 | 0.06 | 2 | 0.12 | mepB of JP4612 was cloned into the expression vector pUCP24 and transformed into E. coli DH5 α |
| pUCP24-mepB/DH5α-JP4200 | 0.06 | 0.5 | 0.12 | mepB of JP4200 was cloned into the expression vector pUCP24 and transformed into E. coli DH5 α |
MIC, minimum inhibitory concentrations; ERV, eravacycline; OMC, omadacycline; TGC, tigecycline; OMC-R, omadacycline-resistant; ERV/TGC-S, eravacycline- and tigecycline-susceptible; Tetracyclines-resistant (eravacycline/omadacycline/tigecycline-resistant); Tetracyclines-susceptible (eravacycline/omadacycline/tigecycline-susceptible).