TABLE 1.
Phenotypic characteristics of K. pneumoniae clinical isolate USA0352/78 (O−:K47) and its derived mutant USA0352/78-3
| Characteristic | Value for strain:
|
|
|---|---|---|
| USA0352/78 | USA03552/78-3 | |
| Mini-Tn5 insertions (no.) | 0 | 1 |
| Capsule productiona | 87 ± 3 | 21 ± 2 |
| C3 binding (NHS)b | 0.3 ± 0.07 | 0.97 ± 0.08 |
| C3 binding (CPS)b | 0.15 ± 0.05 | 0.535 ± 0.05 |
| C3 binding (APS)b | 0.15 ± 0.05 | 0.950 ± 0.015 |
| Phage FC3-10 infection | − | + |
| Phage FC3-11 infection | − | + |
| Complement killingc | 3 × 106/3 × 106 | 3.3 × 106/5 × 104 |
| Opsonophagocytic killingc | 9.3 × 106/5 × 103 | 3 × 106/0 |
a
Cell-associated capsule polysaccharide (femtograms per CFU) quantified by a competitive ELISA.
b
Data are means (in arbitrary A405 units) ± standard deviations from ELISAs done in triplicate at least twice. The results from control wells coated with nonopsonized cells were always less than 0.1 U. CPS, CP serum; APS, AP serum.
c
CFU per milliliter at time (0 h/1 h).