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. 2022 Nov 9;54(11):1901–1912. doi: 10.1038/s12276-022-00886-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a ELISA of conditioned medium from particulate matter-treated cells (CM-PM) immunoprecipitated with control IgG or an anti-HBEGF antibody. b Immunoblot analysis of EGFR phosphorylation in A549 cells after treatment with CM-PM immunoprecipitated with control IgG or an anti-HBEGF antibody. c Wound healing assay of A549 cells treated with CM-PM immunoprecipitated using control IgG or an anti-HBEGF antibody. Images were acquired at 0 and 36 h. Scale bar, 250 μm. d Transwell migration (top) and invasion (bottom) assays of A549 cells treated with CM-PM immunoprecipitated using control IgG or an anti-HBEGF antibody. Scale bar, 200 μm. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001; ns not significant.