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. 2022 Nov 9;54(11):1901–1912. doi: 10.1038/s12276-022-00886-x

Fig. 5. Conditioned medium (CM) from particulate matter (PM)-treated macrophages induces epithelial-to-mesenchymal transition (EMT) in cancer cells.

Fig. 5

a Heatmap of differential gene expression between A549 CM-Control (Con) and A549 CM-PM cells identified by RNA-seq. b Gene set enrichment analysis plot for the “Epithelial-to-mesenchymal transition” gene set from the HALLMARK gene collection. NES normalized enrichment score; FWER familywise error rate; FDR false discovery rate. c Immunoblot analysis of A549 cells incubated for 24 h with the indicated concentration of CM from PM-treated THP1 cells. β-Actin was used as the loading control. d Immunoblot analysis of A549 cells treated with CM-PM immunoprecipitated using control IgG or an anti-HBEGF antibody for 24 h. e qRT‒PCR analysis of A549 cells incubated for 24 h with the indicated concentration of CM from PM-treated THP1 cells. f qRT‒PCR analysis of A549 cells treated with CM-PM immunoprecipitated using control IgG or an anti-HBEGF antibody for 24 h. g Immunofluorescence analysis of A549 cells cultured with CM-PM immunoprecipitated using control IgG or an anti-HBEGF antibody for 24 h. Scale bar, 20 μm. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001; ns not significant.