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. 2022 Nov 9;54(11):1901–1912. doi: 10.1038/s12276-022-00886-x

Fig. 6. Activation of the aryl hydrocarbon receptor (AhR) by particulate matter (PM) in macrophages increases HBEGF expression.

Fig. 6

a Heatmap of the differential expression of AhR-related genes between THP1-Control (Con) cells and THP1-PM2.5, THP1-PM10, and THP1-KRPM cells identified by RNA-seq. b qRT‒PCR analysis of THP1 cells treated with different types of PM (25 μg/cm2) for 24 h. c Immunoblot analysis of PM2.5 (25 μg/cm2)-stimulated AhR translocation. β-Actin and Lamin A were used as the cytoplasmic fraction and nuclear fraction loading controls, respectively. d Immunoblot analysis of THP1 cells treated with the indicated concentrations of PM2.5 for 24 h. e, f qRT-PCR (e) and ELISA (f) of THP1 cells transfected with 50 nM siAhR and treated with 25 μg/cm2 PM2.5 for 24 h. g, h qRT‒PCR (g) and ELISA (h) of THP1 cells pretreated with 10 nM CH-223191 for 1 h and treated with 25 μg/cm2 PM2.5 for an additional 24 h. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001; ns not significant.