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. 2022 Nov 28;54(11):2077–2091. doi: 10.1038/s12276-022-00889-8

Fig. 7. Citratemt accumulation recruits cytoplasmic DRP1 to mitochondria by direct binding to FUNDC1.

Fig. 7

a–d The interaction between FUNDC1 and DRP1 was detected by coimmunoprecipitation (n = 3). a, e Citratemt accumulation upregulated FUNDC1 protein expression (n = 3). f Molecular docking model of FUNDC1-citrate created by AutoDock and PyMOL. FUNDC1-KO MLE12 cells were generated via the CRISPR/Cas9 system. g, h Citratemt accumulation failed to increase the expression of DRP1 or increase the levels of LC3II/LC3I or trimerized MLKL in FUNDC1-KO MLE12 cells; SP-C protein expression was rescued in FUNDC1-KO MLE12 cells (n = 3). The data are shown as the mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001.