Table 2.
MR results of gut microbiota on chronic multisite pain.
| Directional pleiotropy | Cochran Q-test | Rucker’s framework | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Level | Exposure | Outcome | Method | NSNPs | Beta (95% CI) | P | Egger intercept (P) | MRPRESSO global test RSSobs (P) | I2 statistics | Q-statistic (P) | Q’-statistic (P) | Q–Q’ (P) | Steiger P |
| Family | Actinomycetaceae | MCP | IVW | 5 | −0.031 (−0.058, −0.005) | 0.020 | 0.001 (0.804) | 4.299 (0.637) | 0.00% | 2.984 (0.561) | 2.910 (0.406) | 0.074 (0.786) | 2.17E-25 |
| Weighted median | 5 | −0.033 (−0.069, 0.003) | 0.072 | ||||||||||
| MR Egger | 5 | −0.040 (−0.107, 0.028) | 0.331 | ||||||||||
| Genus | Erysipelotrichaceae UCG003 | MCP | IVW | 16 | −0.029 (−0.050, −0.009) | 0.005 | 0.000 (0.879) | 19.059 (0.403) | 4.42% | 15.694 (0.403) | 15.667 (0.334) | 0.027 (0.87) | 5.61E-81 |
| Weighted median | 16 | −0.032 (−0.061, −0.004) | 0.025 | ||||||||||
| MR Egger | 16 | −0.034 (−0.092, 0.025) | 0.275 | ||||||||||
| Order | Actinomycetales | MCP | IVW | 5 | −0.031 (−0.058, −0.005) | 0.021 | 0.001 (0.808) | 4.316 (0.645) | 0.00% | 2.996 (0.559) | 2.926 (0.403) | 0.070 (0.791) | 2.51E-25 |
| Weighted median | 5 | −0.033 (−0.066, 0.000) | 0.050 | ||||||||||
| MR Egger | 5 | −0.040 (−0.107, 0.028) | 0.334 | ||||||||||
| Family | Clostridiaceae1 | MCP | IVW | 10 | 0.031 (0.004, 0.057) | 0.023 | 0.000 (0.926) | 13.584 (0.371) | 0.00% | 7.875 (0.547) | 7.866 (0.447) | 0.009 (0.923) | 9.47E-48 |
| Weighted median | 10 | 0.026 (−0.012, 0.063) | 0.186 | ||||||||||
| MR Egger | 10 | 0.027 (−0.048, 0.102) | 0.497 | ||||||||||
| Order | Gastranaerophilales | MCP | IVW | 9 | 0.024 (0.002, 0.045) | 0.032 | −0.001 (0.811) | 18.611 (0.162) | 34.39% | 12.194 (0.143) | 12.088 (0.098) | 0.106 (0.745) | 1.98E-57 |
| Weighted median | 9 | 0.018 (−0.007, 0.044) | 0.153 | ||||||||||
| MR Egger | 9 | 0.031 (−0.033, 0.095) | 0.371 | ||||||||||
MR, mendelian randomization; MCP, multisite chronic pain; IVW, inverse-variance weighted; NSNPs, number of single nucleotide polymorphisms; Beta, MR effect estimate; CI, confidence interval; RSSobs, residual sums of squares of observations.