Table 2.
Comparison of the characteristics of Bac systems for rAAV production.
| Bac system Taxonomy | Type and name of BEVs | Name of Cell line | Infection efficiency of BEVs | Yield of rAAV (vg/mL) | Versatility and flexibility | Large scale production | System stability |
|---|---|---|---|---|---|---|---|
| ThreeBac [25] | BEV/Rep, BEV/Cap, BEV/(ITR-GOI) | Sf9 | ~6% | 1–5 × 104 | High | Not suitable | Low |
| TwoBac [87] | BEV/Rep-Cap, BEV/(ITR-GOI) | Sf9 | ~27% | 3–9 × 104 | High | Suitable | Medium |
| OneBac [27, 88, 89] | BEV/(ITR-GOI) | Sf9/Rep-Cap | 100% | 1–5 × 105 | Low | Very suitable | Medium to High |
| BEV/Cap-(ITR-GOI) | Sf9-GFP/Rep | 100% | 0.8–2 × 105 | Medium | Very suitable | Medium | |
| BEV/Cap-(ITR-GOI)-Rep | Sf9 | 100% | 1–5 × 105 | High | Very suitable | Medium |