Fig. 4. Anti-IL11 reduces tubular damage, renal fibrosis and preserves kidney function following folic acid-induced acute kidney injury.

a Schematic of X203 therapeutic dosing of FA-injured mice for experiments shown in (b–k). IgG/X203 (10 mg/kg) was administered biweekly starting from day 3 following FA injury. Blood and kidney phenotypes were analyzed on day 28. b Representative kidney gross anatomy, c kidney weights, d Western blots of pERK, ERK, pp90RSK, p90RSK, pGSK3β, GSK3β, SNAI1, ZEB, E-Cadherin, Cyclin D1, αSMA, FN1, and GAPDH (representative dataset from n = 5/group), e representative Masson’s Trichrome images of whole kidney cross section (scale bars: 500 µm, representative dataset from n = 5/group), f quantification of collagen area from Masson’s Trichrome-stained kidney sections (n = 5/group), g kidney collagen contents by hydroxyproline assay, the levels of h BUN, i serum creatinine, and j urine ACRs, k relative mRNA expression of kidney injury (Ngal, Kim1) and pEMT (Snai1, Zeb) markers. c, f–k Data are shown as box-and-whisker with median (middle line), 25th–75th percentiles (box), and minimum–maximum values (whiskers). c, g–k NaHCO₃ (n = 5), FA + IgG (n = 9), FA + X203 (n = 8). c Kruskal–Wallis with Dunn’s correction, f–k one-way ANOVA with Tukey’s correction. FC: Fold change. Source data are provided as a Source data file.