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. 2022 Nov 16;54(11):1991–2006. doi: 10.1038/s12276-022-00875-0

Fig. 3. Lnc Tmem235 inhibits hypoxia-induced apoptosis of BMSCs by regulating BIRC5.

Fig. 3

a mRNA clustering analysis (n = 3); b The expression of BIRC5 mRNA was detected by qPCR (n = 3); baculoviral inhibitor of apoptosis (IAP) repeat-containing 5 (BIRC5); c Western blotting was used to detect the expression of BIRC5 protein (n = 3); d The expression of Lnc Tmem235 was detected by qPCR (n = 3); e The expression of BIRC5 mRNA was detected by qPCR (n = 3); f, g Western blotting was used to detect the expression of BIRC5 protein (n = 3); h The activity of CASP-9 was analyzed by western blotting (n = 3); Caspase-9 (CASP-9); i. The activity of CASP-3 was analyzed by western blotting (n = 3); j The fragmentation of PARP1 was analyzed by western blotting (n = 3); poly (ADP-ribose) polymerase 1 (PARP1); k, l Apoptosis was detected by Annexin V/PI (n = 5); empty vector (GV248); m. The expression of Lnc Tmem235 was detected by qPCR (n = 3); n The expression of BIRC5 mRNA was detected by qPCR (n = 3); o, p The expression of BIRC5 protein was detected by western blotting (n = 3); q, r The activity of CASP-9 was analyzed by western blotting (n = 3); st The activity of CASP-3 was analyzed by western blotting (n = 3); u, v The fragmentation of PARP1 was analyzed by western blotting (n = 3); w, x. Apoptosis was detected by Annexin V/PI (n = 5). In (be, gj, ln, p, r, t, v, x), the data are normally distributed, and the variance is homogeneous. Data are presented as the means ± SDs; in (bc), statistical significance was calculated with Student’s t tests; in (d, e, gj, ln, p, r, t, v, x), statistical significance was calculated by one-way ANOVA with Tukey’s post-hoc tests; *P < 0.05.