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. 2022 Nov 16;54(11):1991–2006. doi: 10.1038/s12276-022-00875-0

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a MiRDB and RNAhybrid predicted the miRNA of the possible combination of Lnc Tmem235 and BIRC5 mRNA 3′UTR; untranslated region (UTR) and microRNA (miRNA); b Binding sites of miR-34a-3p with Lnc Tmem235 and BIRC5 mRNA 3′UTR; c. The expression of miR-34a-3p was detected by qPCR (n = 4); empty vector (GV358); d–f RIP detection of enrichment of Lnc Tmem235 and BIRC5 mRNA to miRNPs (n = 3); RNA-binding protein immunoprecipitation (RIP); g, h Luciferase activity analysis of the binding of miR-34a-3p to Lnc Tmem235 or BIRC5 mRNA (n = 5); i The expression of miR-34a-3p was detected by qPCR (n = 3); j. The expression of Lnc Tmem235 was detected by qPCR (n = 3); k RIP detection of enrichment of Lnc Tmem235 to miRNPs (n = 3); miRNA ribonucleoprotein complexes (miRNPs); l RIP detection of enrichment of BIRC5 mRNA to miRNPs (n = 3); m The expression of BIRC5 mRNA was detected by qPCR (n = 3); n, o Western blotting was used to detect the expression of BIRC5 protein (n = 3). In (c, e–m, o), the data are normally distributed, and the variance is homogeneous. Data are presented as the means ± SDs; statistical significance was calculated by one-way ANOVA with Tukey’s post hoc tests; ^*P < 0.05.