Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 16;54(11):1991–2006. doi: 10.1038/s12276-022-00875-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a At 2 days after operation, the expression levels of GFP and BIRC5 in the femoral head necrotic area were detected by immunofluorescence (n = 6); GFP green fluorescence labeled BMSCs, which could not penetrate bone tissue, green fluorescent protein (GFP), negative control (NC), overexpression (OE), short hairpin (Sh); b At 2 days after surgery, the fluorescence intensity of DiR in the transplantation area was detected by live imaging of small animals (n = 8); DiR red fluorescence labeled BMSCs, which can penetrate bone tissue; c At 2 days after surgery, TUNEL was used to detect apoptosis in the femoral head necrotic area (n = 6); d Quantitative analysis of DiR fluorescence intensity in the transplanted area as shown in b (n = 8); e Quantitative analysis of the proportion of TUNEL positive cells in the transplanted area as shown in c (n = 6); f At 12 weeks post-surgery, H&E staining and Masson staining were used to evaluate the repair of the necrotic area (n = 6); g At 12 weeks after surgery, micro-CT was used to analyze the repair of necrotic area of femoral head (n = 6); h Quantitative analysis of the number of trabeculae as shown in g (n = 6); i Quantitative analysis of the trabecular thickness as shown in g (n = 6); j Quantitative analysis of the volume of new bone tissue as shown in g (n = 6); k Quantitative analysis of the volume fraction of new bone tissue as shown in g (n = 6); l. At 12 weeks post-surgery, the levels of osteogenic markers (e.g., Runx2, OPN, OCN, and OPG) were detected by western blotting (n = 6); osteopontin (OPN); m Quantitative analysis of OPG expression as shown in l (n = 6); n Quantitative analysis of Runx2 expression as shown in l (n = 6); o Quantitative analysis of OCN expression as shown in l (n = 6); p Quantitative analysis of OPN expression as shown in l (n = 6). In (d, e, h–k, m–p), the data are normally distributed, and the variance is homogeneous. Data are presented as the means ± SDs; differences were tested using one-way ANOVA with Tukey’s post-hoc test; ^*P < 0.05.