FIG. 3.

AA release by J774 cells after incubation with β-1,2-oligomannosides. (A) After incorporation of [³H]AA for 2.5 h, radiolabeled cells (5 × 10⁵ cells) were incubated without stimulus (○) or with 0.1 μg of LPS (■) per ml or 25 μM β-1,2-oligomannosides (●). At different time points, [³H]AA release in the cell-free supernatants was measured. (B) Radiolabeled cells were incubated for 18 h with different concentrations of β-1,2-oligomannosides. [³H]AA release in the cell-free supernatants was then determined.