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. 2020 Nov 10;31(2):317–326. doi: 10.4014/jmb.2010.10018

Fig. 4. Effects of antioxidants on MO-in-water emulsion.

Fig. 4

MO-in-water emulsion was prepared with 10% MO, 1% Tween 20, and 1 mM test compounds. MO-in-water emulsion was stored at 35°C for 5 days. (A) Formation of CD was measured on the basis of absorbance at 237 nm. (B) Formation of CT was measured on the basis of absorbance at 270 nm. □, None; ■, 1 mM BHT; ◇, 1 mM VA; △, 1 mM BAVE; ▲, 1 mM TBN; ○, 1 mM CAVE; ●, 1 mM TCN. (C) and (D) CD or CT-forming rate was calculated from the linear equation obtained by the least-square method. (E) Partition behavior of VA, BAVE, and CAVE in MO-in-water emulsion system. Emulsion was prepared with 50% MO, 1% Tween 20, and 1 mM test compounds. After 24 h, emulsion and aqueous phases were analyzed using HPLC.