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Sirt1 and Nrf2 inhibition abolished the cardioprotective and anti-oxidative stress effects of ciprofol in vitro. CK-MB (A), LDH (B) and cTnT (C) activities in each group were detected (n = 6). SOD (C), NOX (D) and MDA (E) levels in each group were assessed (n = 6). ∗p < 0.05 compared to the PBS group. # p < 0.05 as compared to the ISO group. & p < 0.05 compared to the ISO + ciprofol group.
