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The sequences, length, and annealing temperature of primers used for qPCR.
| Target genes | Primer Sequence (5′ →3′) | Size (bp) | Annealing temperature (°C) | Ref |
|---|---|---|---|---|
| icaA | F: 5-GAGGTAAAGCCAACGCACTC-3 | 151 | 62 | |
| R: 5-CCTGTAACCGCACCAAGTTT-3 | ||||
| icaD | F: 5-ACCCAACGCTAAAATCATCG-3 | 211 | 60 | (24) |
| R: 5-GCGAAAATGCCCATAGTTTC-3 | ||||
| 16S rRNA | F: 5GGGACCCGCACAAGCGGTGG-3 | 191 | 60 | |
| R: 5-GGGTTGCGCTCGTTGCGGGA-3 |
