Table 1.
Primers used to confirm and identify serotypes of S. pneumoniae
| Reaction | Serotype/Primer | Primer sequence (5′-3′) | Product size (bp) |
|---|---|---|---|
| 1 | 14-F | CTT GGC GCA GGT GTC AGA ATT CCC TCT AC | 208 |
| 14-R | GCC AAA ATA CTG ACA AAG CTA GAA TAT AGC C | ||
| 19A-F | GTT AGT CCT GTT TTA GAT TTA TTT GGT GAT GT | 478 | |
| 19A-R | GAG CAG TCA ATA AGA TGA GAC GAT AGT TAG | ||
| 19F-F | GTT AAG ATT GCT GAT CGA TTA ATT GAT ATC C | 304 | |
| 19F-R | GTA ATA TGT CTT TAG GGC GTT TAT GGC GAT AG | ||
| 23F-F | GTA ACA GTT GCT GTA GAG GGA ATT GGC TTT TC | 384 | |
| 23F-R | CAC AAC ACC TAA CAC ACG ATG GCT ATA TGA TTC | ||
| 2 | 6A/B-F | AAT TTG TAT TTT ATT CAT GCC TAT ATC TGG | 250 |
| 6A/B-R | TTA GCG GAG ATA ATT TAA AAT GAT GAC TA | ||
| 9V-F | CTT CGT TAG TTA AAA TTC TAA ATT TTT CTA A | 753 | |
| 9V-R | GTC CCA ATA CCA GTC CTT GCA ACA CAA G | ||
| 15A-F | ATT AGT ACA GCT GCT GGA ATA TCT CTT C | 436 | |
| 15A-R | GAT CTA GTG AAC GTA CTA TTC CAA AC | ||
| 15B/C-F | TTG GAA TTT TTT AAT TAG TGG CTT ACC TA | 496 | |
| 15B/C-R | CAT CCG CTT ATT AAT TGA AGT AAT CTG AAC C | ||
| 3 | 1-F | CTC TAT AGA ATG GAG TAT ATA AAC TAT GGT TA | 280 |
| 1-R | CCAAAGAAAATACTAACATTA TCA CAA TAT TGG C | ||
| 3-F | ATG GTG TGA TTT CTC CTA GAT TGG AAA GTA G | 371 | |
| 3-R | CTT CTC CAA TTG CTT ACC AAG TGC AAT AAC G | ||
| 10A-F | GGT GTA GAT TTA CCA TTA GTG TCG GCA GAC | 628 | |
| 10A-R | GAA TTT CTT CTT TAA GAT TCG GAT ATT TCT C | ||
| 11A-F | GGA CAT GTT CAG GTG ATT TCC CAA TAT AGT G | 463 | |
| 11A-R | GAT TAT GAG TGT AAT TTA TTC CAA CTT CTC CC | ||
| 4 | 4-F | CTG TTA CTT GTT CTG GAC TCT CGA TAA TTG G | 430 |
| 4-R | GCC CAC TCC TGT TAA AAT CCT ACC CGC ATT G | ||
| 5-F | ATA CCT ACA CAA CTT CTG ATT ATG CCT TTG TG | 362 | |
| 5-R | GCTCGATAAACATAATCAATATTTGAAAAA GTA TG | ||
| 7C-R | CTATCTCAGTCATCTATTGTTAAAGTTTACGACGGGA | 260 | |
| 7C-R | GAA CAT AGA TGT TGA GAC ATC TTT TGT AAT TTC | ||
| 17F-F | TTC GTG ATG ATA ATT CCA ATG ATC AAA CAA GAG | 693 | |
| 17F-R | GAT GTA ACA AAT TTG TAG CGA CTA AGG TCT GC | ||
| 5 | 7F-F | CCT ACG GGA GGA TAT AAA ATT ATT TTT GAG | 826 |
| 7F-R | CAA ATA CAC CAC TAT AGG CTG TTG AGA CTA AC | ||
| 8-F | GAT GCC ATG AAT CAA GCA GTG GCT ATA AAT C | 294 | |
| 8-R | ATC CTC GTG TAT AAT TTC AGG TAT GCC ACC | ||
| 12A-F | ACT CTT CCA AAT TCT TAT GCT TTT ATT GAT TC | 656 | |
| 12A-R | ATG AAT GAG AAA AGG AAC TTA AAA TTC ATA GC | ||
| 20-F | GAG CAA GAG TTT TTC ACC TGA CAG CGA GAA G | 514 | |
| 20-R | CTA AAT TCC TGT AAT TTA GCT AAA ACT CTT ATC | ||
| 23B-F | TTG TTA GTG GTA TTA AAT TGG GGA CTA CTA GG | 216 | |
| 23B-R | ATA CCT ATC TGA AGT GTT ATT AAC CCA CCA AC | ||
| Positive control | cpsA-F | GCA GTA CAG CAG TTT GTT GGA CTG ACC | 160 |
| cpsA-R | GAA TAT TTT CAT TAT CAG TCC CAG TC |