Table 2.

Summary of nucleic-acid binding to 2C and its effect on ATPase activity

Nucleic acididentity	Dissociation constanta,b, Kd,app (nM)		Fold stimulationckobs (+ssRNA)/kobs (–ssRNA)
NΔ39 2Cb	WT 2C	NΔ39 2C	WT 2C	NΔ39 2C
ssRNA-1	110 ± 20 [70–150]	320 ± 60 [210–440]	32	28
ssDNA-1	110 ± 20 [70–140]	370 ± 90 [170–570]	0.4	0.8
ACE ssRNA-1	70 ± 10 [50–100]	490 ± 100 [280–710]	0.9	1.3
2′-OMe ssRNA-1	70 ± 10 [40–100]	280 ± 50 [190–370]	0.8	1.1
MNA 1	>>> 1000	>>> 2500	0.6	0.7

^aThe apparent dissociation constant (K_d,app) is presented ± standard error with 95% confidence interval provided in brackets below.

^bData from Figure 5.

^cData from Figure 6. Fold stimulation is the quotient of linear rate of RNA-stimulated activity divided by RNA-independent activity.