Fig. 3.

Low-dose E2 inhibits vimentin protein expression by human fibroblast cells. Human fibroblast cell line M1 was treated with 40 ng/mL of IFN-γ or 40 ng/mL of IFN-γ plus 5–50 nM of E2 overnight at 37 °C. (A) Immunofluorescence assay was performed as described in the methods. (B) Relative immunofluorescence intensity was quantitated by ImageJ software (*p < 0.05; Control vs. IFN-γ and IFN-γ vs. E2). (C) Western blot analysis of vimentin using anti-vimentin antibody (ab92547). (D) Densitometric analysis of protein band intensity by ImageJ (*p < 0.05). Data are representative of three separate experiments