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. 2021 Nov 13;1:67. doi: 10.1038/s43705-021-00069-1

Table 1.

Kinetic parameters for D. alaskensis G20 sulfate adenylyl transferase (Sat).

A.
Substrate kcat (s−1) KM (mM) kcat/KM (M−1 s−1) Reference
MoO42− 14.5 ± 1.2 3.26 ± 0.55 4.4 × 103 13
SeO42− 0.284 ± 0.032 1.77 ± 0.55 1.6 × 102 This work
FPO42− 11.0 ± 1.6 1.44 ± 0.56 7.6 × 103 This work
AsO43− 3.88 ± 0.32 0.0617 ± 0.026 6.3 × 104 This work
CrO42− 16.0 ± 0.79 0.793 ± 0.14 2.0 × 104 This work
B.
Inhibitor KI—Varied MoO42− (mM) KI—Varied ATP (mM) Average KI (mM) Reference
ClO3 0.316 ± 0.017 1.45 ± 0.076 0.833 This work
NO3 3.60 ± 0.27 12.5 ± 0.98 8.05 This work
ClO4 0.138 ± 0.014 0.915 ± 0.15 0.5265 13