FIG. 2.

Calcineurin is required for virulence of C. neoformans serotype D and serotype A strains in mice. BALB/c mice (10 for each strain) received injections in the lateral tail vein of 10⁷ cells of the prototrophic CNA1 wild-type MATα serotype D strain (JEC21) or the congenic MATα cna1 mutant strain (MCC2) lacking calcineurin A (A) or the isogenic serotype A CNA1 wild-type (H99), cna1 mutant (AO4), and cna1 plus CNA1 reconstituted (AO10) strains (B). C5-deficient mice (10 each) received injections in the lateral tail vein of 10⁷ cells of prototrophic CNA1 wild-type MATa serotype D strain (JEC20) or the isogenic MATa cna1 mutant strain (MCC10) lacking calcineurin (C) or the prototrophic CNA1 wild-type MATα serotype D strain (JEC21) or the congenic MATα cna1 mutant (MCC5) lacking calcineurin (D). Mice were observed twice daily and the number of mice surviving versus time were plotted.