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. 2022 Nov 12;50(20):11938–11947. doi: 10.1093/nar/gkac1024

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Circuit, repression and binding sites of ArdK. (A) The regulatory circuit of ArdK in plasmid R388. In red, the ardK gene and protein. In green, target promoters for ArdK in the plasmid R388 genome. (B) Transcriptional activity of ArdK target promoters (y-axis) in response to different levels of ArdK induction (x-axis). Dots and error bars represent, respectively, the average and standard deviation (SD) of four independent experiments. Measurements without visible error bars correspond to those with an SD so small that dots and error bars overlap. (C) Location of the transcriptional start sites (+1) and the putative –10 and –35 boxes within the five promoters regulated by ArdK (D) Sequence conservation in ArdK target promoters. Conserved bases are highlighted in grey. Arrows indicate the location of two direct sequence repeats.