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. 2022 Nov 12;50(20):11938–11947. doi: 10.1093/nar/gkac1024

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — (A) Evolution of the KorA/ArdK family. Phylogenetic tree of ArdK/KorA family members showing their experimental (E) or putative (P) operators. The tree was built using the Neighbor–Joining algorithm, and numbers on the branches indicate bootstrap values. (B) ArdK is able to repress DRs with different spacers. Transcriptional activity of a synthetic promoter containing a TTGACA DR, with both arms separated by the number of bases indicated in the key. Promoter activity, expressed as the percentage of the maximum GFP/OD₆₀₀ value (y-axis), is plotted against the ArdK induction level (x-axis). Dots and error bars represent, respectively, the average and SD of three independent experiments. (C) ArdK is able to repress IRs. Transcriptional activity of a synthetic promoter containing a TTGACA IR, separated by a loop of variable length, as shown in the key. Promoter activities and induction levels are expressed as above.