Figure 4.

Analysis of class II suppressor double mutant alternative splicing on select introns. (A) Reverse transcription and PCR reactions for alternative splicing. Strains are indicated at top and alternative splicing products and sequences of 5′ss regions are indicated at right. The results of three biological replicates of the double suppressor strains are shown. (B) Quantitation of alternative splicing results of the indicated suppressor strains. Percent usage of the upstream 5′ss for each intron in each strain is shown, along with the standard deviation and number of trials. Quantitation of the splice products are found in Supplementary Table S1.