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. 2022 Nov 2;50(20):11834–11857. doi: 10.1093/nar/gkac991

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Analysis of alternative splicing of two additional prp-8(D1549) mutant alleles. New strains tested include a D1549D control allele from the CRISPR experiment, and the new viable alleles D1549I and D1549V. RNA was isolated from the indicated strains and the alternative 5′ splicing events in mab-10, Y71H2AM.2 and tofu-7 analyzed. Those strains with >20% ΔPSI in this experiment compared to the control on the left are indicated with a blue star (average of two independent experiments, see Supplementary Table S1 for quantitation data). An asterisk on the right of the mab-10 gel denotes a spurious background band that is not reproducible for prp-8(D1549I).