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. 2022 Aug 10;50(20):11401–11414. doi: 10.1093/nar/gkac641

Figure 5.

Figure 5.

A single dose of FORCE–M23D is sufficient to restore dystrophin localization to the sarcolemma in skeletal and cardiac muscles of mdx mice. Five-week-old mdx mice were injected via tail vein with vehicle, 30 mg/kg unconjugated M23D, or 10 mg/kg or 30 mg/kg PMO-equivalents of FORCE–M23D and sacrificed at the indicated time points. (A) Representative immunofluorescence images with dystrophin (green) and laminin (red) staining of quadriceps cross-sections isolated 4-, 8- and 12-weeks post-dose from vehicle-treated WT or mdx mice, or from mdx mice treated with 30 mg/kg PMO-equivalent of FORCE–M23D. (B) Quantification of dystrophin positive fibers in quadriceps of mdx mice. (C) Representative immunofluorescence images with dystrophin (green) and laminin (red) staining of diaphragm (C) and heart (D) cross-sections isolated 4-, -8-weeks post-dose from vehicle-treated mdx mice or from mdx mice treated with 30 mg/kg PMO-equivalent of FORCE–M23D. Data represent mean ± SD. **** P < 0.0001. PMO, phosphorodiamidate morpholino oligomer; WT, wild type.