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. 2022 Nov 22;2:117. doi: 10.1038/s43705-022-00195-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Fractional abundances of microbial classes in the microbiomes of mesopelagic animals based on A 16S rRNA amplicon data and B metagenomic data. Gammaproteobacteria, Bacteroidia, Bacilli and Clostridia were the most prevalent bacterial classes across microbiomes based on 16S rRNA gene profiling (see also Table 2). These classes were also recovered through shotgun metagenomic sequencing, although with lower abundance. By contrast, a markedly higher proportion of Actinobacteria was revealed in each sample through this method (paired t-test: t = –8.2128, p < 2.548e^–07).