Fig. 4. Proportional abundance heatmap based on Euclidean distance for metabolic functions predicted from gene-level shotgun metagenomic data.

Samples were clustered with the complete linkage method, where similarity between clusters is determined by the distance between the most dissimilar members of the respective clusters. Functional potential shows only weak associations with diel vertical migration status and diet, but is partly linked to host taxon and depth as indicated by hierarchical clustering between samples and PERMANOVAs (Table 4). Note that genes might belong to multiple metabolic categories.