Table 4.

PERMANOVAs assessing abundance of metabolic categories in relation to host species, depth, diet and diel vertical migration based on a 16S rRNA amplicon data and b shotgun metagenomic data.

Source of variation	df	SS	R2	p	pDispersion
16S rRNA data
Host	10	2.5220	0.4591	0.001	0.157
Residuals	26	2.9712	0.5409
Diet	2	0.5843	0.1064	0.027	0.114
Residuals	34	4.9089	0.8936
Migration	2	0.5892	0.1073	0.025	0.300
Residuals	34	4.9041	0.8928
Depth	4	1.0597	0.1929	0.012	0.007
Residuals	32	4.4335	0.8071
Metagenomic data
Host	9	0.1357	0.6726	0.001	0.008
Residuals	22	0.0661	0.3274
Diet	2	0.0497	0.2463	0.004	0.030
Residuals	29	0.1521	0.7537
Migration	2	0.0344	0.1706	0.024	0.001
Residuals	29	0.1674	0.8295
Depth	3	0.0620	0.3073	0.003	0.017
Residuals	28	0.1398	0.6927

Data were transformed into Bray–Curtis dissimilarities for analysis. P values for permutational MANOVA and permutation tests for homogeneity of multivariate dispersions are shown. Significant tests (p ≤ 0.05) are indicated in bold.

df degrees of freedom, SS sum of squares, R² coefficient of determination (proportional explained variation), p p value.