Fig. 2. Relative abundances for phyla present at greater than 1% abundance in at least one sample.

A) 16 S rRNA gene amplicons; B) 16 S rRNA genes derived from the assembled metagenomes; C) concatenation of 16 syntenic ribosomal proteins derived from the assembled metagenomes, with scaffold coverage as a proxy for abundance; and D) high quality bins (containing the 16 concatenated ribosomal proteins, and with abundances calculated from the ribosomal protein-encoding scaffolds’ coverages). The Composite Leachate Cistern at timepoint 1 (CLC_T1) is represented by the 0.2 µm filter’s sequence information, as the 0.1 µm filter showed highly similar results. Site GW2, the unimpacted groundwater well, did not yield sufficient biomass for metagenomic sequencing, thus only 16 S rRNA gene amplicons are reported here.