Table 2.
Test results from gls models indicating the significance of correlation between temperature variables and bacterial diversity, including the abundance of main (>5% of total reads) potentially pathogenic (PP) genera.
| Months | Date | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Skin | Cold/warm months | T_month mn | T_month SD | T14_mn | T14_SD | T4_mn | T4_SD | T7_mn | T7_SD | T2_mn | T2_SD |
| Shannon | 21 (0.0001) | 21 (0.0001) | 48.8 (0.0001) | 46.6 (0.001) | 28 (0.0001) | 55 (0.0001) | 96 (0.0001) | 29 (0.0001) | 31 (0.0001) | 38 (0.0001) | 7 (0.01) |
| Shannon SD | 21 (0.0001) | 102 (0.0001) | 367 (0.0001) | 224 (0.0001) | 940 (0.0001) | 183 (0.0001) | 663 (0.0001) | 3 (0.1) | 28 (0.0001) | 16 (1–4) | 21 (0.0001) |
| PD | 32 (0.0001) | 25 (0.0001) | 38 (0.0001) | 45 (0.0001) | 14 (2-4) | 58 (0.0001) | 72 (0.0001) | 22 (0.0001) | 36 (0.0001) | 27 (0.0001) | 1.5 (0.2) |
| PD SD | 6 (0.01) | 155 (0.0001) | 379 (0.0001) | 223 (0.0001) | 233 (0.001) | 224 (0.0001) | 427 (0.0001) | 3 (0.1) | 28 (0.0001) | 16 (1-4) | 21 (0.0001) |
| Unifrac weighted | 151 (0.0001) | 169 (0.0001) | 146 (0.0001) | 164 (0.0001) | 71 (0.0001) | 230 (0.0001) | 187 (0.0001) | 191 (0.0001) | 48 (0.0001) | 324 (0.0001) | 45 (0.0001) |
| Unifrac unweighted | 532 (0.0001) | 562 (0.0001) | 450 (0.0001) | 653 (0.0001) | 102 (0.0001) | 673 (0.0001) | 369 (0.0001) | 704 (0.0001) | 196 (0.0001) | 634 (0.0001) | 18 (0.0001) |
| Aliivibrio | 0.6 (0.4) | 0.8 (0.4) | 5 (0.02) | 3 (0.1) | 10 (0.002) | 1 (0.3) | 5 (0.03) | 0.9 (0.4) | 5 (0.02) | 0.9 (0.4) | 2 (0.2) |
| Photobacterium | 2 (0.2) | 9 (0.003) | 4 (0.04) | 16 (0.0001) | 18 (0.0001) | 7 (0.01) | 2 (0.2) | 2 (0.1) | 0.3 (0.6) | 0.6 (0.4) | 8 (0.01) |
| Pseudomonas | 306 (0.0001) | 564 (0.0001) | 248 (0.0001) | 736 (0.0001) | 37 (0.0001) | 711 (0.0001) | 90 (0.0001) | 839 (0.0001) | 19 (0.0001) | 850 (0.0001) | 3 (0.1) |
| Vibrio | 3 (0.1) | 1 (0.3) | 1 (0.3) | 5 (0.03) | 0.7 (0.4) | 3 (0.1) | 0.7 (0.4) | 13 (3-4) | 5 (0.02) | 9 (0.003) | 3 (0.1) |
| Gill | |||||||||||
| Shannon | 87 (0.0001) | 61 (0.0001) | 93 (0.0001) | 91 (0.0001) | 33 (0.0001) | 111 (0.0001) | 146 (0.0001) | 81 (0.0001) | 17 (0.0001) | 106 (0.0001) | 2 (0.2) |
| Shannon SD | 483 (0.0001) | 908 (0.0001) | 1886 (0.0001) | 1379 (0.0001) | 302 (0.0001) | 1136 (0.0001) | 2030 (0.0001) | 60 (0.0001) | 115 (0.0001) | 67 (0.0001) | 354 (0.0001) |
| PD | 88 (0.0001) | 76 (0.0001) | 104 (0.0001) | 110 (0.0001) | 44 (0.0001) | 129 (0.0001) | 139 (0.0001) | 97 (0.0001) | 17 (0.0001) | 115 (0.0001) | 1 (0.3) |
| PD SD | 182 (0.0001) | 820 (0.0001) | 3347 (0.0001) | 1189 (0.0001) | 210 (0.0001) | 651 (0.0001) | 1773 (0.0001) | 60 (0.0001) | 115 (0.0001) | 67 (0.0001) | 354 (0.0001) |
| Unifrac weighted | 223 (0.0001) | 230 (0.0001) | 148 (0.0001) | 225 (0.0001) | 89 (0.0001) | 265 (0.0001) | 140 (0.0001) | 244 (0.0001) | 30 (0.0001) | 255 (0.0001) | 24 (0.0001) |
| Unifrac unweighted | 624 (0.0001) | 658 (0.0001) | 498 (0.0001) | 736 (0.0001) | 103 (0.0001) | 787 (0.0001) | 397 (0.0001) | 709 (0.0001) | 134 (0.0001) | 651 (0.0001) | 28 (0.0001) |
| Pseudomonas | 236 (0.0001) | 446 (0.0001) | 172 (0.0001) | 461 (0.0001) | 498 (0.0001) | 554 (0.0001) | 43 (0.0001) | 720 (0.0001) | 15 (2–4) | 1014 (0.0001) | 5 (0.03) |
| Vibrio | 0.1 (0.7) | 0.02 (0.9) | 0.5 (0.5) | 0.5 (0.5) | 3 (0.1) | 0.4 (0.5) | 1 (0.3) | 3 (0.1) | 23 (0.0001) | 2 (0.2) | 8 (0.004) |
| Water | |||||||||||
| Shannon | 0.1 (0.8) | 4 (0.1) | 6 (0.02) | 20 (0.0001) | 0.3 (0.6) | 9 (0.003) | 0.3 (0.6) | 6 (0.02) | 0.1 (0.8) | 6 (0.01) | 0.1 (0.8) |
| PD | 0.6 (0.4) | 0.03 (0.9) | 1 (0.2) | 0.5 (0.5) | 0.6 (0.4) | 0.04 (0.9) | 0.04 (0.9) | 0.004 (0.9) | 0.0002 (0.9) | 0.4 (0.6) | 0.6 (0.4) |
| Unifrac weighted | 5 (0.03) | 3 (0.1) | 3 (0.1) | 4 (0.1) | 1 (0.5) | 4 (0.1) | 1 (0.3) | 2 (0.2) | 1 (0.3) | 2 (0.2) | 0.3 (0.6) |
| Unifrac unweighted | 25 (0.0001) | 29 (0.0001) | 14 (2–4) | 45 (0.0001) | 1 (0.3) | 39 (0.0001) | 6 (0.01) | 31 (0.0001) | 6 (0.01) | 35 (0.0001) | 0.1 (0.7) |
Correlation structure was estimated based on distance matrices for both dependent and independent variables using the corMLPE R package. For each test we report the F statistic and significance (P value with significant correlations indicated in bold). Months were separated into cold and warm based on whether the mean monthly temperature was lower or higher than the daily annual average (i.e., 18.6 °C). T_month_mn: mean monthly temperature; T_month_SD: standard deviation of T_month_mn; T14_mn: mean temperature of the 7 days previous to both sampling dates in a month; T14_SD: standard deviation of T14_mn; T4_mn: mean temperature from the 2 days previous to both sampling dates in a given month; T4_SD: standard deviation of T4_mn; T7_mn: mean temperature from the 7 days previous the sampling date; T7_SD: standard deviation of T7_mn; T2_mn: mean temperature from the 2 days prior to the sampling date; T2_SD: standard deviation of T2_mn.