TABLE 2.
Distribution of mtsa-10 and esat-6 among mycobacteria
| Species and strain | Presence ofa:
|
|
|---|---|---|
| mtsa-10 | esat-6 | |
| M. tuberculosis W | + | + |
| M. tuberculosis H37Ra | + | + |
| M. tuberculosis H37Rv | + | + |
| M. tuberculosis CDC 1551 | + | + |
| M. africanum | + | + |
| M. bovis NADL | + | + |
| M. bovis Ravenel | + | + |
| M. bovis Branch | + | + |
| BCG Pasteur | − | − |
| BCG Japan | − | − |
| BCG Connaught | − | − |
| BCG Montreal | − | − |
| BCG Russia | − | − |
| M. asiaticum | − | − |
| M. avium | − | − |
| M. fortuitum | − | − |
| M. gastri | + | + |
| M. haemophilum | − | − |
| M. kansasii | + | + |
| M. malmoense | − | − |
| M. phlei | − | − |
| M. scrofulaceum | − | − |
| M. simiae | − | − |
| M. terrae | − | − |
| M. triviale | − | − |
| M. ulcerans | − | − |
+, presence of hybridization signal; −, absence of hybridization signal. Mycobacterial strains were cultured at 36°C in an atmosphere containing 5% CO2 on Middlebrook 7H10 (Difco) agar plates containing 0.5% (vol/vol) glycerol and supplemented with 10% (vol/vol) albumin-dextrose-catalase. An exception was M. haemophilum, which was cultured on chocolate-agar plates at 30°C. The culture medium for M. africanum was supplemented with 0.5% (wt/vol) pyruvic acid. Cells were harvested after 4 days of culturing for fast-growing mycobacteria and 4 weeks of culturing for slow-growing mycobacteria. DNA isolation and Southern transfer hybridization were performed according to a standard protocol employed in DNA fingerprinting of M. tuberculosis (28).