Figure S2.

Senescent cells inhibit macrophages’ ability to phagocytose. (A) Schematic overview of the experimental design to monitor phagocytosis by macrophages in co-culture with proliferating or senescent cells. 16 h after the assembly of the co-cultures, either Escherichia coli, secondary necrotic corpses or inert silicon beads were added. Uptake by macrophages was monitored over time using the IncuCyte S3 system. (B) Removal of the labeled phagocytotic prey by macrophages was quantified over time. Macrophages were in co-culture with either proliferating (black line) or senescent cells (red line) of the indicated origin.