Subject	Molecular biology
Specific subject area	Transcriptomics
Type of data	Small RNA-sequencing datasets of three Mycobacterium tuberculosis strains, i.e., SBH49, SBH149, and SBH372, susceptible to streptomycin, isoniazid, rifampicin, and ethambutol.
How data were acquired	Illumina HiSeq 2500 sequencing platform
Data format	Raw data (fastq)
Description of data collection	The bacteria were isolated from sputum samples and cultured in Middlebrook 7H9 broth using BACTECTM MGITTM 320. Total RNAs were extracted from the pure culture and subjected to small RNA sequencing.
Data source location	•Region: Southeast Asia •State: Sabah •Country: Malaysia •District: Kota Kinabalu (SBH49), Penampang (SBH149), and Semporna (SBH372)
Data accessibility	Repository name: NCBI Sequence Read Archive Data identification number: SRX16744291 (SBH49), SRX16744292 (SBH149), SRX16744293 (SBH372) Direct URL to data: https://www.ncbi.nlm.nih.gov/sra/PRJNA863377