FIGURE 3.

Sequence alignment of ancestral enzymes and representative sequences of PLK/HMPPK‐like and HMPPK enzymes. Tt‐HMPPK (Thermus thermophilus), St‐HMPPK (Salmonella typhimurium), Ec‐HMPPK (Escherichia coli), Sa‐PLK/HMPPK (Staphylococcus aureus), Bs‐PLK/HMPPK (Bacillus subtilis) and Mc‐PLK/HMPPK (Macrococcus caseolyticus). Secondary structure annotation corresponds to the AncC structure. The proposed residues that determine substrate specificity are highlighted in red. Blue asterisks indicate a new set of active site residues involved in ligand recognition