Table 1.

Inhibition of Pdr5 ATPase activity by transport substrates

Substrate	WT	V1372A	I685A	V1372A, I685A
Clotrimazolea	1.17 (0.29)	1.96 (0.38)	1.78 (0.15)	6.33
Bifonazole	2.86	3.29	2.58	13.0

^aThe IC₅₀ values (μM) for clotrimazole were determined from assays of two independently prepared sets of PM vesicles for WT, V1372A, and I685A. The averages and SD in parentheses are shown. Only one determination was made for the double mutant at high enough concentrations to obtain the IC₅₀ (Fig. S1).