Table 2.

Major phenotypic features of the gating region mutants

Mutanta	Drug specific?	ATPase activity	R6G transport against/with gradient deficient?	Sensitivity to allostery
S1368Ab	No	WT	Yes/No	WT
F1369A	No	WT	Yes/No	WT
F683L	No	WT	Yes/No	WT
M1373T	Yes	WT	NDc	WT
A684S	Yes	WT	ND	WT
V1372A	Yes	WT	No/No	hyperresistant
I685A	Yes	WT	Yesd/ND	hyperresistant
G1371A	No	Reduced	Yes/Yes	hypersensitive

^aThe G682A and M1373A mutant proteins are absent from PM vesicles and these mutants are not listed.

^bData are from Mehla et al. (13).

^cNot determined.

^dVery mild, but statistically significant.