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. 2022 Nov 7;298(12):102681. doi: 10.1016/j.jbc.2022.102681

Figure 2.

Figure 2

MITF regulates STIM1 promoter activity in melanocytes.A, schematic representation of the αMSH-induced melanogenesis via cAMP-PKA-CREB-MITF signaling axis. B, normalized luciferase activity of STIM1 promoter in B16 cells upon 1 μM αMSH treatment for 48 h (N = 4). C, normalized luciferase activity of STIM1 promoter upon overexpression of either KCREB or VP16-CREB in B16 cells (N = 3). D, normalized luciferase activity of STIM1 promoter upon MITF overexpression in B16 cells (N = 3). E, normalized luciferase activity of STIM1 promoter upon MITF overexpression in HEK293T cells (N = 3). Data presented are mean ± S.E.M. For statistical analysis, one sample t test was performed for panels B, D, and E, while one-way ANOVA followed by Tukey’s post hoc test was performed for panel C. F and p values for panel C are F (2, 6) = 153.9 and p < 0.0001, respectively. Here, NS means nonsignificant; ∗p <0.05; ∗∗p < 0.01, and ∗∗∗∗p < 0.0001. CREB, cAMP response element binding protein; cAMP, cyclic AMP; MITF, Microphthalmia-associated transcription factor; STIM1, Stromal Interaction Molecule1; αMSH, α-Melanocyte Stimulating Hormone.