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. 2022 Dec 1;10(12):e005651. doi: 10.1136/jitc-2022-005651

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Screening for IFNγ secretion after stimulation with cryptic peptides and neoantigens. pTc and TiTc of HROC113 (A, B) and HROC285 (C, D) were stimulated with peptide pools for 28 and 14 days, respectively. Number of spots was normalized to controls. N=2–3, Significance was assessed by t-test. Striped columns: peptides with weak binding prediction; color-filled columns: peptides with strong binding prediction; orange columns: significantly increased IFNγ release (p<0.05, determined by t-test). pTc, peripheral blood Tc; TiTc, tumor-infiltrating Tc.