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. 2022 Dec 1;10(12):e005651. doi: 10.1136/jitc-2022-005651

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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T cells specific for neoantigens and cryptic peptides. At days 0 and 14 of stimulation pTc and TiTc of HROC113 were stained with tetramers specific for the neoantigen IQGAP1neo (A, B) and pTc and TiTc of HROC285 with tetramers specific for the neoantigen TRIT1neo (C, D). The amount of Tc specific the cryptic peptides #8, #18 and #32 was determined in pTc and TiTc HROC113 stimulated with B-LCL loaded with a pool of immunogenic cryptic peptides (stim) or without any peptide (ctrl) (E, F). B-LCL, B-lymphoblastoid cells; pTc, peripheral blood Tc; TiTc, tumor-infiltrating Tc.