Figure 6.

Flow cytometry to explore the influence of BiTP on the TME in the 4T1-hPD-L1 tumors. (A–C) The gating strategies for CD8⁺ T cell, NK cell, and DC in flow cytometry assays. (D-T) The quantification of tumor-infiltrating lymphocyte, CD8⁺ T cell, CD69⁺ CD8⁺ T cell, CD25⁺ CD8⁺ T cell, Ki67⁺ CD8⁺ T cell, Granzyme-B⁺ CD8⁺ T cell, Perforin⁺ CD8⁺ T cell, TNF-α⁺ CD8⁺ T cell, NK cell, Ki67⁺ NK cell, Granzyme-B⁺ NK cell, IFN-γ⁺ NK cell, Perforin⁺ NK cell, TNF-α⁺ NK cell, DC, CD80⁺ DC, and CD86⁺ DC. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 mean the significant difference compared with BiTP-treated samples. DC, dendritic cell; TME, tumor microenvironment.