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Solvent gradient for the determination of antibiotics by high-performance liquid chromatography.
| Retention time (min) | Flow rate (mL/min) | Mobile phase A (water liquid chromatography-mass spectrometry grade) (%) | Mobile phase B (acetonitrile with 0.1% (v/v) formic acid) (%) |
| 0 | 0.80 | 95 | 5 |
| 1 | 0.80 | 95 | 5 |
| 12 | 0.80 | 70 | 30 |
| 13 | 0.80 | 0 | 100 |
| 17 | 0.80 | 0 | 100 |
| 17.1 | 0.80 | 95 | 5 |
| 23 | 0.80 | 95 | 5 |
