Figure 5.

Inhibition of A3B_CTD-QM-ΔL3-AL1swap-catalyzed deamination of 5′-T₄CAT by dZ-containing linear and cross-linked oligos. Experimental conditions are 400 μM of 5′-T₄CAT, 8 μM of dZ-containing oligos, 300 nM of A3B_CTD-QM-ΔL3-AL1swap in a 50 mM sodium phosphate buffer (pH 6.0) containing 100 mM NaCl, 2.5 mM β-mercaptoethanol, 0.1 M EDTA, and 50 μM 3-(trimethylsilyl)-2,2,3,3-tetradeuteropropionic acid at 25°C. K_i value of linear dZ-oligo on A3B_CTD-QM-ΔL3-AL1swap has been established previously by our group.¹⁹