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| oligo | parameters | linear analysis (Lineweaver–Burk plot) | non-linear regression analysis |
|---|---|---|---|
| 5′-AT3CAT3 | Km, μM | 200 ± 30 | 150 ± 30 |
| Vmax, μM/s | 0.014 ± 0.002 | 0.0126 ± 0.0008 | |
| kcat, s–1 | 0.29 ± 0.04 | 0.26 ± 0.02 | |
| kcat/Km, s–1 μM–1 | 0.0014 | 0.0018 | |
| 5′-T4CAT | Km, μM | 350 ± 70 | 290 ± 70 |
| Vmax, μM/s | 0.056 ± 0.008 | 0.051 ± 0.005 | |
| kcat, s–1 | 0.19 ± 0.03 | 0.171 ± 0.015 | |
| kcat/Km, s–1 μM–1 | 0.00054 | 0.00059 |
