Table 3. Inhibition Constants (K_i) for dZ-Containing Oligo Inhibitors of A3B_CTD-QM-ΔL3-AL1swap Obtained by Various Calculation Methods^a.

oligo name	parameters	Lineweaver–Burk plot for substrate/Dixon plot for inhibitor	non-linear regression analysis for both substrate and inhibitor
dZ-linear	Ki, μM	7.5 ± 1.7	7.0 ± 1.3
	Vmax, μM/s		0.0126 ± 0.0008
dZ[UE(−2), AN3(+1)]X	Ki, μM	0.66 ± 0.14	0.69 ± 0.14
	Vmax, μM/s		0.052 ± 0.006

^adZ-linear inhibitor was evaluated against 5′-AT₃CAT₃ substrate using 50 nM of A3B_CTD-QM-ΔL3-AL1swap; dZ[U^E(−2), A^N3(+1)]X was evaluated against 5′-T₄CAT substrate using 200 nM of A3B_CTD-QM-ΔL3-AL1swap.